Fecha/hora: 01/06/2024 13:37:34

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	29/07/2020	
Fecha de la última actualización: 	05/09/2022	
Autoria: 	CASTELLS, M.; CAFFARENA, D.; CASAUX, M.L.; SCHILD, C.; MIÑO, S.; CASTELLS, F.; CASTELLS, D.; VICTORIA, M.; RIET-CORREA, F.; GIANNITTI, F.; PAREÑO, V.; COLINA, R.	
Afiliación: 	MATÍAS CASTELLS BAUER, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay./INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Facultad de Veterinaria, Universidad de la República, Alberto Lasplaces 1620, Montevideo 11600, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SAMUEL MIÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires 1686, Argentina.; FELIPE CASTELLS, Doctor en Veterinaria en Ejercicio Libre, Asociado al Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.; DANIEL CASTELLS, Centro de Investigación y Experimentación Dr. Alejandro Gallinal, Secretariado Uruguayo de la Lana, Ruta 7 km 140, Cerro Colorado, Florida 94000, Uruguay.; MATIAS VICTORIA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIVIANA PAREÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires 1686, Argentina; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.	
Título: 	Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.	
Año de publicación: 	2020	
Fuente/Imprenta: 	Pathogens (Basel, Switzerland), July 2020, Volume 9, Issue 7, Article number 570, Pages 1-17. OPEN ACCESS. DOI: https://doi.org/10.3390/pathogens9070570	
DOI: 	10.3390/pathogens9070570	
Idioma: 	Inglés	
Notas: 	Article history: Received: 7 April 2020 / Revised: 20 June 2020 / Accepted: 30 June 2020 / Published: 14 July 2020.
This research was funded by Instituto Nacional de Investigación Agropecuaria (INIA), grant number PL_015 N-15156 and N-23398, and by the Universidad de la República  Program Polo de Desarrollo Universitario. The APC was funded by Universidad de la República.	
Contenido: 	Abstract:
Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.	
Palabras-Clave: 	BOVINE; DIARRHEA; GENOTYPES; INTERSPECIES TRANSMISSION; PLATAFORMA SALUD ANIMAL; ROTAVIRUS.	
Thesagro: 	GANADO BOVINO.	
Categoría del tema: 	--	
URL: 	http://www.ainfo.inia.uy/digital/bitstream/item/16692/1/pathogens-09-00570.pdf
https://www.mdpi.com/2076-0817/9/7/570/pdf	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103160-1) - PXI - AP - DD - PP/Pathogens/2020 -  -  - DIS